I've been using TweetDeck now for several months. Except for its propensity to swallow memory, I think it's probably one of the best Twitter interfaces out there.
If you don't already use it, I'd highly recommend you give TweetDeck a go.
Recent updates have extended the service further to offer Facebook integration and other nice features like TwitPic previews.
One of the useful features I use on Tweetdeck daily is the facility to set up groups.
Groups allow me to follow defined collectives of Twitter users for specific reasons.
Amongst my groups I have "work colleagues", "web PM's" and "SEO" to name a few.
Whenever a member of a group posts a tweet it alerts you via the usual pop-up message, but defining which group has posted.
If you've got some work relating to a specific sector or project area its very hand for staying in touch with a subject as it develops.
TweetDeck will create a new column in the application window that displays any posts that your group members post. Making it far easier to view the tweets from group members.
Once you're finished amending your group, simply save via the button at the bottom and the new group takes effect immediately.
TweetDeck also offers a similar functioning search facility, but I think I'll save that for another day!
Groups are a great way of sifting through all the information that is quickly passing before your eyes.
They allow you to focus on campaign related conversations or follow particular individuals and their updates, ensuring you never miss out on trends or developing discussions again.
Enjoy TweetDeck groups!
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